The nitrogen fixing efficiency of Rhizobium is highly influenced by the agroelimatic conditions prevailing at a particular place. The present work deals with the comparative study of nitrogen fixing efficiency of imported recommended strains-viz., VR 169, VR 441, VR 420 and RCR 3824 and local isolates GKP3, GKP4, GKP5. N2-fixing efficiency of isolates/strains was studiedin terms of parameters like nodule number, nodule dry weight, activity of nitrogenase (N2-ase) and biomass in Vigna mungo. Local isolates GKP3, GKP4 and GKP5 were found better among the 10 isolates collected from the nodules of local wild legumes. The findings of the present study revealed that local isolate GKP3 was most potent N2-fixer.