Germination precentage, growth of plumule and radicle and accumulation of non-photosynthetic pigments were studied in three cultivars of mungbean exposed to two levels of UV-B radiation. All three varieties were found to be sensitive to UV-B radiation in terms of reduction in seedling growth. The magnitude of the response was however, different. Reduction in plumule growth was more than the radicle indicating the sensitivity of plumule to UV-B radiation more than the radicle. Cultivar PS-16 was found to be most senstive to UV-B radiation. Germination percentage was largely unaffected by UV-B radiation. Non- photosynthetic pigment content was found to decrease in seedlings exposed to UV-B radiation accompanied by low white light, indicating the inhibition of photoreactivation leading to UV-B damage.