In Vitro Propagation of Rose Cultivars

Nodal explants (1.5–3 cm) were grown on MS medium containing various levels of IAA and BAP. Multiple shoots were produced on medium containing 2.0 mg/1 BAP and 0.2 mg/1 IAA. After 4 weeks of culture the condition of shoots of cv. Contempo deteriorated due to yellowing of leaflets. Addition of AgNO₃ in the medium resulted in reduction of leaf abscission as well as enhanced growth of the axillary shoots. The in vitro grown propagules were rooted in 1/4 MS media supplemented with 0.1 mg/1 IAA + 0.1 mg/1 IBA. Rooting was evident in 8 cultivars where as two cultivars did not respond. The resultant plantlets were acclimatized and grown in potted soil where they developed normally.