Plant regeneration from cotyledonary node of Punica granatum L.

Multiple shoots were induced within 10 days, in 100% cotyledonary node explants of in vitro grown seedlings of Punica granatum L., on B5 medium supplemented with 0.05 mg I⁻¹ NAA + 1 mg I⁻¹ kinetin + 1 mg I⁻¹ benzyl adenine in cv. Ganesh and 0.05 mg I⁻¹ NAA + 0.5 mg I⁻¹ BA in cv. Kabul. The multiplication factor was 7–9 per subculture per month. Shoots of both the cv. were elongated on b5 medium supplemented with 0.05 mg I⁻¹ NAA+0.5 mg I⁻¹ BA + 10% coconut milk. 45 days old shoots were rooted on B5 medium containing 3 mg I⁻¹ IAA for 2 months. Plantlets were hardened in net house having 14000–17000 lux light, at temperature below 30° and RH 80% for 2 months. All plants survived during acclimatization and flowered within 16 months after transfer to field.