Department of Plant Physiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, Uttaranchal, India.
Callus induction and plant regeneration from mature embryo was studied in six indica rice cultivars, namely, Pusa Basmati 1, Basmati 370, Type 3, CSR 10, Pant Dhan 4 and Pokkali. Maximum callus initiation was observed on MS medium supplemented with 2 mg l−1 2,4-D (2, 4-dichlorophenoxy acetic acid) in all the cultivars. Wide variation in callus growth was observed amongst the varieties tested. MS medium without 2, 4-D but supplemented with different concentrations of BAP and IAA was used to evaluate the regeneration efficiency. 0.5 mg. l−1 BAP was found to be optimum for regeneration of plantlets in all the cultivars.
Callus proliferation, genotypic variation, plant regeneration, rice
