Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110 012.
1Present address and address for correspondence: Division of Biotechnology, I.I.H.R., Hessarghatta Lake Post, Bangalore - 560 089.
Efficiency of protocorm like bodies (plbs) production as a function of various growth regulators was assessed in three orchid genera viz. Dendrobium ‘sonia’, Oncidium ‘Gower Ramsay’ and Cattleya leopoldii. Growth regulators viz. BAP, kinetin, NAA, IAA, 2,4-D and GA3 were supplemented to MS medium at concentrations of 0.25, 0.5 and 1.0 mg l−1. The number of days taken for initial differentiation was observed to be a function of growth regulator supplemented as well as orchid genera. Maximum number of plbs were produced in BAP 1.0 mg l−1. The relative ranking among plant growth regulators (highest to lowest) for obtaining maximum number of plbs was BAP>Kinetin>NAA>IAA>2,4-D>GA3. Similar ranking for three orchid genera was Dendrobium>Oncidium>Cattleya.
Growth regulators, orchids, protocorm like bodies
