Micropropagation of the halophyte Suaeda nudiflora Moq. through axillary bud culture

An in vitro micro propagation protocol has been developed for the halophyte Suaeda nudiflora Moq. Nodal explants were used for axillary shoot proliferation. The best results for shoot multiplication were recorded on MS medium suplemented with BAP (1.0 mg l⁻¹) and KN (0.2 mg l⁻¹). The MS medium supplemented with BAP in combination with auxins resulted very few shootlets and profuse callusing of the explants. Half strength MS liquid medium supplemented with IAA + IBA + NAA + IPA (0.5 mg l⁻¹) produced rooting in 70% of the in vitro regenerated shoots. Further root development was achieved in half strength MS agar medium without any auxins in two weeks. PlantIets were established on sterile vermiculite under controlled conditions and later transferred to pots or plastic bags containing 1: 1 mixture of soil and sand.