Optimization of bapand iaa concentration on shoot induction, proliferation and rooting in shoot-tip culture of banana cv. dwarf cavendish

The effect of BAP (6-benzylaminopurine) and IAA (indole-3-acetic acid) alone and in combination was investigated on shoot initiation, multiplication and root induction for Dwarf Cavendish banana. Shoottips (1–1.2 cm) were isolated from field collected suckers to initiate the culture. Concentration of BAP ranged from 0–6 mg L⁻¹ for shoot induction and from 0–8 mg L⁻¹ for shoot multiplication. The concentration of IAA in both initiation media and multiplication media ranged from 0–2 mg L⁻¹. The explants responded best on shoot induction media containing MS media supplemented with BAP (6 mg L⁻¹) and IAA (1 mg L⁻¹). Highest multiplication rate was observed on the same medium. Best root induction and proliferation was observed on half strength MS media supplemented with 1 mg L⁻¹ IAA.