In vitro micropropagation of Baliospermum axillare blume

An efficient method of direct plantlet regeneration using nodal explants of mature plants of Baliospermum axillare has been developed for the first time. Nodes were cultured on Murashige and Skoog medium (MS) containing indole-3-butyric acid (IBA) at 1 and 2 mg/l in combination with 6-benzylaminopurine (BAP) at 1, 2 and 5 mg/l. A maximum number of 15 multiple shoots per explant were obtained on MS medium supplemented with 2 mg/l BAP and 1 mg/l IBA. The regenerated shoots rooted in MS basal media. The plantlets were maintained in vermiculite and soil mixture and were successfully transferred to soil.