Enhanced plant regeneration from cotyledonary node explants of mungbean by amino acids

In mungbean (Vigna radiata (L.) Wilczek), shoot buds were induced when cotyledonary node explants were supplemented with benzylaminopurine (10 μM). When benzylaminopurine (BAP, 5 μM)and napthaleneacetic acid (NAA, 0.05 μM)were supplemented to the medium, 30–35 per cent of induced buds developed into micro shoots. Supplementation of amino acids like glutamine, proline and L-cysteine at the concentration of 40 and 80 mg dm⁻³ to the shoot bud development medium resulted in enhanced regeneration. The fresh mass increased with higher concentration of amino acids whereas number of micro shoots decreased. Ethylene and methane evolution was observed during the regeneration process. There was an increase in methane evolution and reduction in ethylene evolution with the addition of amino acids.