Shoot bud regeneration from leaf explants of Albizzia amara Boiv.

High frequency plant regeneration was achieved from the callus of leaf explants of Albizzia amara Boiv. Optimum callus was achieved on Murashige and Skoog medium supplemented with 3.5 mg/l of α-naphthalene acetic acid (NAA) and 0.5 mg/l of 6-benzylaminopurine (BAP). Subsequent culturing of callus on the same medium containing various concentrations and combinations of auxins and cytokinins, resulted in shoot bud induction. Maximum number of shoot buds were produced on the medium supplemented with 3.5 mg/l NAA and 1.5 mg/l BAP. Further growth and development was achieved on hormone free MS medium. The regenerated shoots were rooted on ½ strength MS medium containing 0.6 mg/l indole-3 butyric acid (IBA) and the plantlets were hardened and transferred to soil in the open. Anatomical studies revealed that shoot bud differentiation was observed from sub epidermal level as well as in the center of the callus.