Efficiency of thidiazuran on in vitro shoot regeneration from cotyledonary node explant in mungbean

Mungbean (Vigna radiata (L.) Wilczek) is an important pulse crop and shows recalcitrant behaviour in vitro cultures. The present study reports the shoot regeneration of cotyledonary node explants of mungbean grown on thidiazuran (TDZ) enriched MS medium. The shoot bud development was enhanced by 8 days exposure of explants to 2 mM TDZ concentration. The complete loss of regeneration ability and high callus growth of explants was observed at higher concentration of TDZ and with continuous exposure of explants to TDZ.