Induction of chlorophyllase activity in normal green and chlorotic leaves of sugarcane

Influence of supplementation of micronutrients (Fe, Cu and Zn) at the concentration in which they occur in the leaves of normal green plant on the activity of chlorophyllase enzyme was examined in normal green and chlorotic leaves of sugarcane variety CoH 92 (Saccharum spp. hybrid, ratoon crop) grown at the Kharika block of IISR, Lucknow. It was found that the enhancement of chlorophyllase activity was more in chlorotic leaf substrate as compared to normal green leaf substrate when iron, copper and zinc were supplemented to the chlorotic leaf enzyme preparation. In the case of normal leaf enzyme preparation, supplementation of Fe and Cu were found to enhance the activity of chlorophyllase only when the normal leaf substrate was used.