Cloning of Acetyl-CoA carboxylase gene from Brassica Juncea cv. Pusa bold
Abstract
Using degenerate primers, a partial genomic sequence encoding ACCase gene was PCR amplified, cloned and analyzed for sequence homology. Using this partial sequence as probe and on screening genomic library of B. juncea, two putative positive clones were isolated and confirmed by restriction and Southern analysis. A ~5 kb fragment, identified by Southern blot hybridization was subcloned which showed strong hybridization signal with probe pACC1. This confirmed the cloning of ACCase gene of B. juncea.
Keywords
ACCase (acetyl-CoA carboxylase), cloning, genomic library screening, PCR amplification, subcloning
