Indian Journal of Plant Physiology
  • Year: 2004
  • Volume: 9
  • Issue: 2

Biochemical characterisation of gliadins in wheat

  • Author:
  • Natique Siddique, Archana Sachdev, R.P. Johari
  • Total Page Count: 7
  • Page Number: 105 to 111

Division of Biochemistry, Indian Agricultural Research Institute, New Delhi - 110 012

*Corresponding author; E-mail: arcs_bio@yahoo.com

Abstract

Wheat varieties, viz. C-306, HD-2745, HD-2735 (good chapati quality) and Sonalika (poor chapati quality) were selected for the characterization of soluble, storage and gliadin protein. SDS-PAGE pattern of soluble, storage and gliadin showed only qualitative changes among the varieties. PAGE pattern of gliadins was also similar. RP-HPLC analysis of these varieties showed significant differences in the relative concentration of major/minor peaks.

Keywords

Gliadin, quality, storage, protein, wheat