Nature and mode of regeneration in cotyledonary nodal explants of pigeonpea

In vitro shoot regeneration and histological studies were conducted to understand the nature and mode of regeneration in cotyledonary nodal explants of pigeonpea (Cajanus cajan (L.) Millsp.) cv. Pusa 855 under the influence of BAP. It was observed that presence of pre-existing axillary bud was absolutely necessary for the multiple shoot bud induction and regeneration involved direct organogenesis without any intermediary callus phase. Dedifferentiation occurred from the sub-epidermal cells of the basal parts of pre-existing axillary bud.