Department of Botany, University of Pune, Pune - 411 007
*Corresponding author; E-mail: mgs@unipune.ernet.in
Nodal sector (3 cm) and axillary bud (1 cm diameter) explants of field grown grape (Vitis vinifera L.) cv. Tas-A-Ganesh aseptically cultured on Murashinge and Skoog's (MS) basal medium containing 0.0 to 24.2 μM BAP, 0.0 to 34.2 μM IAA, 0.0 to 29.54 μM IBA, 0.0 to 22.6 μM 2, 4-D and 0.0 to 26.8 μM NAA separately and in various combinations. Eighty and eighty five per cent of cultures showed callusing at 19.72 μM NAA and 9.04 μM 2, 4-D, respectively. The maximum growth of callus of weight 1.987 g and 1.825 g per explant was produced at 10.2 μM NAA and at 9.04 μM 2,4-D, respectively, indicating these concentrations to be optimum for the growth of the callus. All cultures produced callus at very high rate using a combination of 2.22 μM of BAP + 10.72 μM NAA, indicating that combination of BAP and NAA is best for induction of callus and callus growth where 2.15 g of callus produced per explant. Multiple shoots induced with 2.2 to 22.0 μM BAP from both the explants. Maximum number of shoots (3.7 ± 0.48 per explant) were obtained at 13.2 μM BAP alone. However, combination of 11.0 μM BAP + 2.85 μM IAA induced healthy and stout shoots (4.1 ± 0.31 per explant), indicating it is the best hormone combination than supplementing BAP alone. In vitro grown shoots (2.0 to 8.0 cm height and 30 days old) when transferred to MS medium supplemented with increasing concentrations (0.0 to 34.2 μM) of IAA and (0.0 to 29.52 μM) IBA separately, 93 and 99% cultures induced rooting at 5.7 μM IAA and 4.92 μM IBA, respectively. Rooting at all concentrations of IBA was superior than IAA in this experiment. IBA (4.92 M) was the best for rooting of in vitro plantlets. Fifty one per cent of in vitro grown plantlets were hardened at 80 to 85% humidity and transferred to field successfully.
Callus, grape, multiple shoots, rooting
