Indian Journal of Plant Physiology

In vitro fibre development was initiated in cotton (G. hirsutum L.) cv LRA 5166 by culturing three day o fertilized ovules. The ovules were cultured on standard basal mediums (MS & TM) containing different hormonal combinations. Fibre initiation took place when isolated ovules were cultured in suspension or semi-solid medium under dark incubation. Ovules responded to changes in media constituents and hormon by producing fibres or de-differentiating into callus, and in certain treatments both callus and fibres we observed simultaneously. The best result was observed in ovules cultured on TM medium with IAA (1.0 mg l⁻¹) + GA (1.0 mg l⁻¹) + BA (1.0 mg l⁻¹) for 15 days and later sub-cultured on TM medium with IAA (2.0 mg l⁻¹) + BA (0.5 mg l⁻¹) with a fibre length of 15–17mm in 25–30 days of culture.