Somatic embryogenesis in grape (Vitis vinifera L.) CV. Tas-A-Ganesh from leaf explants

Embryogenic calli developed in the leaf discs of field grown grapevine (Vilis vinifera L.) cv. Tas-A-Ganesh, cultured on MS medium supplemented with various combinations of BAP (0.0–17.6 μM), TDZ (2.25–4.5 μM) and NOA (5–25.00 μM). Among these combinations, 17.6 μM BAP+4.5 μM TDZ+20 μM NOA combination was the best for embryo production when transferred to MS medium supplemented with 2.2 μM BAP +1 μM IAA (where 26% of calli showed embryogenesis). Somatic embryos were photographed and were confirmed by microtomy. These embryos germinated on hormone-free MS medium. Percentage of germination was however, very poor (9.0%).