Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar, 125004.
*Corresponding author, E-mail: vijayc@hrau.ernet.in
Plant regeneration protocol from callus cultures of chickpea cultivars was developed using cotyledonary node, shoot tip, leaflet, internode and hypocotyl as explant source. Except leaflet explant, all the explants used showed high degree of callus induction on B5 basal medium supplemented with BAP (1.5 mg/l) and NAA (1.0 mg/l). MS medium supplemented with TDZ (1 mg/l) was found suitable for callus induction in leaflet explants. For callus induction as well as for shoot regeneration, cultivar HC-I showed higher response than cultivars C-235 and H86–18. Regenerated shoots were rooted on half strength MS medium. The hardened regenerated plantlets were finally transferred to soil. However, survival percentage of the plantIets was low (11%) in soil.
Cicer arietinum L., shoot morphogenesis, thiadiazuron (TDZ)
