$All India Networking Project on Betelvine, Bapatla-522 101, Andhra Pradesh, India
†Acharya Nagarjuna University Post-Graduate Centre (ANUPGC), Nuzvid-521 201, Andhra Pradesh, India
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502 324, Andhra Pradesh, India
*Paper presented in the National Conference on Organic waste utilization and ecofriendly technologies for crop protection during March 2007.
Polyclonal antibodies were raised against purified polyhedrin [(polyocclusion body (POB)] protein preparations of Helicoverpa armigera nucleopolyhedrovirus (HaNPV) and were used to develop a direct antigen coating enzymelinked immunosorbent assay (DAC-ELISA) to detect HaNPV. The sensitivity of the DAC-ELISA was 15 ng/ml of partially purified viral protein or 30 ng/ml POBs from the HaNPV infected larval extracts. The antibodies were highly specific to polyhedrin protein of HaNPV and were useful to diagnose NPV at early stages of larval infection and also for the quantification of the NPVs during production of viral insecticides.
Polyclonal antibodies, Helicoverpa armigera, nucleopolyhedrovirus, ELISA
