Induction of Defense Enzymes in Trichoderma viride Treated Blackgram Plants in Response to Macrophomina phaseolina Infection

Efficacy of various Trichoderma viride isolates were tested for the management of dry root rot of blackgram caused by Macrophomina phaseolina. In greenhouse studies, seed treatment plus soil application of talc based formulation of T. viride isolates Tv¹and Tv⁴along with FYM effectively reduced the disease incidence and increased the yield. Expression of various defense related enzymes was found involved in the induction of systemic resistance against pathogen infection. Blackgram seed treated with 4 g kg^-1plus soil application 4 g kg^-1of talc based formulation of T. viride (Tv¹and Tv⁴) compared with FYM with challenge inoculation of M. phaseolina enhanced the induction of defense enzymes such as peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL).