Indian Journal of Plant Protection
  • Year: 2013
  • Volume: 41
  • Issue: 4

Serodiagnosis of Latent Infection of Ralstonia solanacearum in Potato

  • Author:
  • Rashid Khan, Jameel Akhtar1,, Virendra Singh Pundhir, Anil Kumar2, Z Khan1
  • Total Page Count: 4
  • Page Number: 338 to 341

1Divion of Plant Quarantine, National Bureau of Plant Genetic Resources, New Delhi - 110 012, New Delhi, India

2Department of Molecular Biology & Genetic Engineering, GBPUA&T, Pantnagar - 263 145, Uttarakhand, India

Department of Plant Pathology, GB Pant University of Agriculture & Technology, Pantnagar - 263 145, Uttarakhand, India

*E mail: jakhtar@nbpgr.ernet.in

Online published on 5 June, 2014.

Abstract

Anti-potato and anti-tomato polyclonal antibodies were used for serodiagnosis to detect latent infection of Ralstonia solanacearum infecting potato. Maximum latent infection in tubers was observed in local variety (Tumdi) followed by cv. Kufri Giriraj and Kufri Jyoti whereas minimum latent infection was found in Tumdi at seedling stage than other varieties. The absorbance recorded at seedling stage showed least population of bacteria, even though tubers have considerable amount of inoculum.

Keywords

Immunodiagnosis, potato, indirect ELISA, Bacterial wilt