Indian Journal of Plant Protection
  • Year: 2015
  • Volume: 43
  • Issue: 3

Biological and biochemical characterization of isolates of Helicoverpa armigera Nucleopolyhedrovirus [HaNPV] from different geographic locations of India

  • Author:
  • K Sireesha, Ch Sreedhar Kumar1, G V Ranga Rao1, P Lava Kumar2
  • Total Page Count: 7
  • Page Number: 305 to 311

1Entomology Unit, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502 324, Hyderabad, Telangana State, India

2Virology and Molecular Diagnostics UnitInternational Institute of Tropical Agriculture (IITA), PMB-5 320, Ibadan, Nigeria

Horticultural Research Station (ARI), Lam, Guntur-522 034, Andhra Pradesh, India

*E mail: sirisha_ento@yahoo.co.in

Online published on 21 December, 2015.

Abstract

Six strains of HaNPV collected from different places of India were compared for their biological and biochemical characteristics. Based on the bioassay tests against second and third instar larvae of Helicoverpa armigera the order of activity in increasing order is UASD-HaNPV< AK-HaNPV<TN-HaNPV<PAU-HaNPV<GAU-HaNPV<ICRISAT-HaNPV and no correlation was drawn between biological and biochemical characteristics. Electron microscopic observations of polyhedra, alkali disrupted polyhedra during purification and nucleocapsids are also presented.

Keywords

Nucleopolyhedrovirus, structural polypeptides, biological characterisation, Helicoverpa armigera