Toxicity of biorational insecticides and their effect on some biological aspects of Helicoverpa armigera
Abstract
A study was undertaken to evaluate toxicity of six biorational insecticides against Helicoverpa armigera (Hubner) under laboratory conditions. In leaf dip bioassay, on the basis of LC50values against 3rdinstar larvae of H. armigera, the order of toxicity of tested insecticides was emamectin benzoate > azadirachtin > spinosad > Bacillus thuringiensis > B. bassiana > HaNPV. The respective LC50values for these insecticides were 0.00015, 0.00016, 0.00143, 0.00144 and 0.00277 per cent. The LC50value for HaNPV was 2.39 x 105PIB ml-1. However, field doses calculated on the basis of LC50were recorded with highest cumulative mortality of 3rdinstar larvae in emamectin benzoate 5 SG (60.5%) and spinosad 45 SC (56.2%). For per cent pupation of surviving larvae, emamectin benzoate (22.3%) and spinosad (26.6%) showed significantly lowest pupation. Similar pattern was observed in pupal weight and healthy pupae. Maximum adult emergence was in control (90.0%) followed by HaNPV (37.2%). Azadirachtin 1 EC had the latent effect on hatchability of eggs laid by recovered adults by significantly reducing egg hatching. In ovicidal tests, lowest hatchability of eggs 72 h after treatment was recorded with spinosad (23.6%) and emamectin benzoate (30.5%) followed by B. thuringiensis (43.3%), B. bassiana (68.3%), HaNPV (70.4%) and azadirachtin (78.1%), respectively.
Keywords
Bioassay, biorational, biological parameter, emamectin benzoate 5 SG, Helicoverpa armigera, spinosad 45 SC