Indian Journal of Poultry Science

Web of Science
  • Year: 2004
  • Volume: 39
  • Issue: 1

Estimation of parental genomic proportion in specialized chicken population using marker assisted introgression*

  • Author:
  • D. Anil Pavan Kumar, R.K.S. Bais, Deepak Sharma, R.B. Prasad
  • Total Page Count: 5
  • DOI:
  • Page Number: 49 to 53

Department of Genetics and Animal Breeding, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar-263 145 (U A).

Abstract

Despite of poor production, the indigenous birds have important characteristics, particularly thc major genes which have large effect on the productive ability under tropical climatic conditions. The crossing of native Naked neck donor line possessing such characteristics with the high yielding recipicnt line, followed by repeatcd back crossing with the recipient line for several generations is a traditional and easy way of introgressing the desired characteristics. Studies have shown that genetic markers can be used in introgression ofgene and is called Marker Assisted Introgression.

A total of 10 RAPD primers were screened to detect polymorphism among the BC2 population and grand sire using pooled DNA samples of BC2 progenies and grand sire. The parental genomic proportion from the donor grand sire to the BC2 progenies was estimated in terms of the genetic similarity between the grand sire and BC2 progenies. The genetic similarity between grand sire and BC2 progenies was calculated as average band sharing (BS) proportion between the grand sire and Be2 progenies which ranged from 0.476 to 0.923. The genetic distance (DS) between the grand sire and BC2 progenies ranged from 0.080 to 0.740. These results were seen within as well as between the BC2 population. The effect of parental genomic proportion between High, Medium and low parental genomic proportion were non-significant for these traits.

Keywords

RAPD, Marker assisted introgression, Naked neck