Indian Journal of Poultry Science

Web of Science
  • Year: 2006
  • Volume: 41
  • Issue: 1

Detection of infectious bursal disease virus (IBDV) from bursal tissue by RT-PCR and its comparative efficacy with conventional precipitation assays

  • Author:
  • N.R. Makadiya, M.K. Jhala1, Amit Gaba, C.G. Joshi2, D.N. Rank2
  • Total Page Count: 3
  • DOI:
  • Page Number: 82 to 84

Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand - 388 001 (Gujarat)

Abstract

The Infectious bursal disease virus (IBDV) causes considerable morbidity and mortality mainly by immunosuppression in chicken. There are reports of emergence of very virulent IBDV (vvIBDV) strains in different parts of the world including India during the last couple of decades. The present study was aimed at screening IBD suspected bursal samples by reverse transcription-polymerase chain reaction (RT-PCR) and conventional precipitation assays viz. agar gel immunodiffusion (AGID), and counter immunoelectrophoresis (CIE) to compare their relative sensitivity and specificity. RNA was extracted by Tri Reagent® method and cDNA was prepared with Omniscript™ Reverse Transcriptase Kit. VP2 gene specific primers (P1, P2) were used for generating 643 bp amplification. Out of 37 samples, sensitivity of AGID and CIE compared to RT-PCR was 74.19 and 87.10 per cent and overall agreement was 78.38 and 89.19 per cent respectively.

Keywords

Infectious bursal disease virus, AGID, CIE, RT-PCR