Hatching observation in ex-vivo embryo culture system and normally incubated eggs

The study was conducted on a sample size of 233 fertile eggs of IC-3, medium body size broiler populations aged between 32 to 35 weeks, obtained through natural mating with the purpose of improving efficiency of ‘Double Window Ex-vivo Chick Embryo Culture System at Phase II and III. Consequently, 134 eggs from the same strain were used as control. Out of 233 eggs set, 97 chicks were hatched out and out of 134 control eggs, 112 chicks came out on 22^nd day. Hatching observations were done in both the groups. Hatching observation with respect to yolk sac, allantoic fluid, urates, beak, orientation of embryo, feet-leg visibility, membrane penetration, breathing and activity of embryos in ex-vivo embryo culture system were recorded. The observations were studied in terms of Pipped (pipped but could not hatch), Hatched (total number of hatch out) and Navel (connected/detached) in control group. The results concluded that for successful hatching of embryos in culture system, the orientation of embryos should be on left position, visibility of right feet/legs, horizontal position of beak, chorioallantoic membrane and yolk sac are becoming attached, climax activity and dry allantoic fluid. These optimum conditions with relation of position of embryo in embryo culture system should be maximized through suitable modifications in the procedure for healthy hatching.