Indian Journal of Poultry Science

Web of Science
  • Year: 2007
  • Volume: 42
  • Issue: 3

Cloning of fowl adenovirus-4 DNA in a plasmid vector

  • Author:
  • M. Rai, S.M. Singh, A. Rai
  • Total Page Count: 3
  • DOI:
  • Page Number: 299 to 301

Department of Animal Science, MJP Rohilkhand University, Bareilly - 243 006, (UP).

Abstract

The DNA was isolated from fowl adenovirus-4 recovered in India and digested with restriction enzyme PstI and BamHI. The digested viral DNA was analyzed by 0.7% agarose gel electrophoresis. Digestion of FAV-4 DNA with PstI enzyme produced seven fragments of 17.38, 11.80, 4.62, 3.79, 3.27, 2.02 and 1.75 kb while digestion with BamHI gave eight fragments of 12.65, 11.22, 7.5 4.48, 3.35, 3.05, 1.92 and 1.75 kb sizes. Some bands were not visible in lower portion. The average size of FAV-4 DNA was 45 kb.The PstI and BamHI digested DNA fragments were cloned in pUC18 cloning vector.

Keywords

Virus, cloning, DNA, fowl adenovirus-4, restriction enzyme analysis