Indian Journal of Poultry Science

Web of Science
  • Year: 2007
  • Volume: 42
  • Issue: 3

PCR Amplification of penton gene of fowl adenovirus - 4

  • Author:
  • M. Rai, S.M. Singh, A. Rai
  • Total Page Count: 2
  • DOI:
  • Page Number: 304 to 305

Department of Animal Science, MJP Rohilkhand University, Bareilly - 243 006, (UP).

*Corresponding author: E-mail: raia48@gmail.com

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Abstract

FAV-4 penton gene (1578 bp) was successfully amplified using self-designed specific primers using FAV-4 DNA as template and cycling conditions of initial denaturation at 94°C for 3 min followed by 30 cycles of denaturation at 94°C for 1 min, annealing at 56°C for 1 min and elongation at 72°C for 2 min and a final extension at 72°C for 5 min. The amplified DNA run on 0.7% agarose gel electrophoresis along with MW marker showed a single DNA band of 1578 bp size.

Keywords

Virus, Penton gene, fowl adenovirus, cloning