PCR-RFLP of growth hormone gene in meat type chicken

In this study, growth hormone gene polymorphism has been investigated in meat type chicken by PCR-RFLP. Three different profiles (AA, CC and AC genotypes) created by a combination of polymorphic MspI cut sites due to A+119G in intron-1 were identified. The genotype amplicons (AA, CC) were sequenced and submitted in the genebank with accession No. EF472954 and EF452679. The frequency of the CC (64.8%) genotype was higher in meat type (PB-1) chicken. The analysis of the identified SNP with growth traits showed that A+119G was significantly associated with body weight at 5 weeks of age in meat type chicken. On phylogenetic analysis, the Gallus gallus nucleotide sequences clustered into a monophyletic group closer to Meleagris gallopavo and farthest from Cairina moschata.