Indian Journal of Poultry Science

An experiment was conducted to see the effect of dietary supplementation of phytase and gainase on haemato-biochemical constituents, immunological response, bone mineral content, weight gain and economics of raising Ven Cobb broiler chicken. Day old broiler chicks (250) were divided into five treatment groups having five replicates of ten chicks each viz.,T₁, control, T₂, negative control, TT and T supplemented with phytase (250g/ton), gainase (500g/ton) and phytase + gainase 250 + 500g/ton), respectively. Diet for pre starter (1–14 d) contained 23% protein, 3000 (T₁) and 2900 kcal/kg (T₂-T₅) ME. During starter (14-28d) phase diet contained 21.5% protein and ME content was 3150 (T₁) and 3050 kcal/kg diet (T₂-T₅). Diets for finisher (28-42d) birds contained 20% protein and 3250 (T₁), 3150 kcal/kg (T₂-T₅) ME, respectively. The available phosphorus content during all three phases was 0.45, 0.35, 0.35, 0.45, and 0.35% in T₁ to T₅, respectively. Supplementation of enzyme did not have any significant effect on haematological and biochemical parameters amongst different treatment groups, however, these enzymes increased the activity of aspartate amino transferase and reduced the alkaline phosphatase and alanine amino transferase activity. Serum P was significantly (P<0.05) higher in T₅ group. These enzymes had no significant effect on serum HI titers against Newcastle disease virus, on weight of lymphoid organs and liver. The ash, Ca and P content of tibia and toe bone significantly (P<0.05) increased in T₃. Enzyme supplementation significantly increased Zn, Mn and Fe content in tibia and toe. The net profit for raising broiler chicken up to 42 d was higher in T₅ (Rs 11.71/kg) as compared to T₂ (Rs 9.93) and control (Rs 10.22). It is inferred that supplementation of phytase and gainase in a low energy and available P diet had a positive, economical and growth boosting effect on raising of broiler chicken.