Study of TGF-β₃ gene in synthetic White Leghorn chicken

A total of 250 pullets of Synthetic White Leghorn were housed at 16 weeks of age in individual California cage system and various important economic traits were recorded on individual birds. On the basis of feed consumption and egg production during 51–60 weeks, these birds were categorized i.e., High feed consumption and High egg production (HH), Low feed consumption and High egg production (LH), High feed consumption and Low egg production (HL) and Low feed consumption and Low egg production (LL) groups. From each group 10 birds were utilized for the present study. Genomic DNA was extracted from these selected 40 birds by John's method with some modification. Chicken gene specific primers (20 bp) were used for amplification of TGF-β₃ gene and amplicon was digested with Bsel-I RE for RFLP analysis. There was absence of polymorphism, as TGF-b₃ gene for all the samples were found to be monomorphic.