Biofilm production and Multi Drug-Resistance (MDR) pattern of Paratyphoid Salmonella isolated from poultry

The present study was carried out to determine the prevalence of Multi Drug-Resistant (MDR) paratyphoid bacteria in poultry. The study was conducted for a period of eight months, from July 2019 to February 2020 and a total of 26 poultry farms including 15 broiler and 11 layer farms, were visited and pooled faecal samples were obtained. A total of 189 necropsy cases were examined. The prevalence of salmonellosis in the broiler and layer farms was recorded as 20.0% and 45.4% respectively through microbe Out of total 189 necropsy cases of birds, salmonellosis was recorded in 1.58% birds. Polyvalent antisera tested 18.18% isolates positive for Salmonella Enteritidis while 9.09% tested positive for Salmonella Typhimurium. The PT bacteria were found to be multi-drug resistant (MDR) where resistance was observed in the motile Salmonella isolates towards oxytetracycline (100%), streptomycin (66.6%) and amoxicillin (33.3%), while 66.6% of motile paratyphoid Salmonella isolates were found resistant for two or more than two antibiotics. Biofilm assay was performed using crystal violet method and 40.0% isolates possessed biofilm producing ability out of which 20.0% isolates were weak biofilm producers and 20.0% isolates were moderate biofilm producers. The finding is of much importance since biofilm formation contributes to both virulence and antibiotic resistance of the bacteria.