Age related changes in PPARγ, COX III mRNA expression, growth performance, carcass quality parameters and gut morphometry in different genotypes of chicken

Tarai, S., Thyagarajan, D., Srinivasan, G. and Mangalagowri, A. 2024. Age related changes in PPARγ, COX III mRNA expression, growth performance, carcass quality parameters and gut morphometry in different genotypes of chicken. Indian Journal of Poultry Science, 59(1): 49–57.

Efficient conversion of food into body mass abide associated with altered gene expression of some proteins of the electron transport chain. We evaluated the outcome of age on mRNA expression of Peroxisome proliferator-activated receptor-y and Cytochrome c oxidase subunit III in breast muscle tissues. Collectively 90 birds each from Nandanam B2, Rhode Island Red, Aseel and White Leghorn, divided into three replicates were accostomed for the study. Production parameters like body weight, body weight gain, cumulative feed consumption, cumulative feed efficiency at two and six weeks were documented along with carcass traits at eight weeks and intestinal morphometry were studied at four and eight weeks of age. Total RNA was drawn out from the breast muscle tissue of male birds and reverse transcribed into cDNA. Real-time PCR analysis was executed using specific primers for the genes. Greatest reduction when comparing second and sixth week old was noted in COX III mRNA expression levels followed by PPARγ. The study revealed phenotypic differences in production traits as well as the difference in expression of mitochondrial gene like COX III and nuclear encoded gene that is involved in fat metabolism like PPARγ gene expression level change with age in chickens.