International Journal for Research in Applied Sciences and Biotechnology(IJRASB)
  • Year: 2020
  • Volume: 7
  • Issue: 6

Transferring of hybrid f1 of spring wheat into dihaploid basis using andgrogen thechnologies

Junior Teaching Assistant, Department of Agronomy, Faryab University, Afghanistan

*Corresponding Author: aqarahimwasim@gmail.com

Online published on 3 February, 2021.

Abstract

Anther culture is the most technologically advanced method of obtaining doubled haploids. Dihaploid lines are required to speed up the breeding process. From 7 hybrids of first-generation (F1) of spring wheat, 15 dihaploid culture lines were obtained by anther culture. An average of 23.7 embryo-like structures was obtained on 150 anthers. The percentage of plant regeneration was 49.1%, of which there were 39.9% albino plants and 9.1% green plants. The percentage of spontaneous chromosome doubling among the obtained green plants was 35.7.

Keywords

Anthogenesis, Anther Culture, Embryogenesis, Regeneration