Biotechnological Protein Profiling Study in Wild Chickpea Treated with Ethyl Methane Sulphonate

The genus Cicer belongs to the family Papilionaceae (Leguminosae) with 9 annual species and 31 perennial species, of which only one of 9 annuals species is under cultivation-Cicer arietinum. The level of polymorphism is very low within the cultigen as has been reported by many workers. The available genetic variation has been utilized in the breeding programmes which culminate into narrowing of the genetic base for this crop. The seed storage protein profile by SDS-PAGE Electrophoresis has been studied in wild chickpea treated with chemical mutagens EMS in M3 generation. The comparative analysis on the basis of Jaccard's similarity coefficient matrix and UPGMA clustering has been studied. The banding pattern revealed polymorphism in the present investigation with 7 to 12 major band while other minors in control and EMS-mutants. The polypeptide bands of various sizes ranging from 6.13 KDa to120.24 KDa were observed in all the treatments alongwith the control parent. The genetic distance between all 5 treatments varied from 0.2 to 0.6 as reveled by Jaccard similarity coefficient matrix. The Cluster analysis separated control and EMS-mutants into different group and genetic distance observed between them low.