Application of Response Surface Methodology for optimization of Biomass, Carbohydrate and Lipid production in BG11⁺ by Scenedesmus quadricauda

In the present communication response surface methodology (RSM) was applied on BG11⁺ medium to optimize the biomass, carbohydrate and lipid in Scenedesmus quadricauda. In first set of experiments; effect of various macronutrients of BG11⁺ medium were defined towards biomass, carbohydrate and lipid production using the factorial design. The NaNO₃, K₂HPO₄, MgSO₄·7H₂O and pH were resulted as the important factors affecting the optimal growth, carbohydrate and lipid production of S. quadricauda. In second set of experiment, the levels of NaNO₃, K₂HPO₄, MgSO₄·7H₂O and pH in growth medium was optimized using the RSM whilst other parameter were kept constant as in original BG11⁺ medium. The biomass, carbohydrate and lipid content in original BG11⁺ medium were 1.10 gL⁻¹, 161 μg mL⁻¹ and 28% (w/w) respectively whilst in RSM optimized medium biomass, carbohydrate and lipid contents were 1.94 gL⁻¹, 450 μg mL⁻¹ and 48%(w/w) respectively.