Experimental challenge of white spot syndrome virus (WSSV) From Polychaete Pereneris cultifera To Peppermint shrimp Lysmata wurdemanni in captivity

White Spot Syndrome Virus (WSSV), a large double-stranded DNA virus, is the major and most serious pathogen in the shrimp aquaculture industry. White spot syndrome virus (WSSV) collected from Polychaete-Perinereis cultrifera which was orally transmitted with white spot syndrome virus (WSSV) and conformed with PCR identification is transferred to peppermint shrimp Lysmata wurdemanni by intramuscularly injection and through oral rout.100% mortality of L. wurdemanni was resulted on 19th day in oral rooted and on 21st day for intramuscularly injected shrimp. The PCR analysis observed the appearance of a prominent band of PCR amplified product of WSSV-DNA at 848 bp at 24 hrs to 8 day of WSSV post-inoculum to the pleopods of L. wurdemanni. Whilst these band 650 bp, 296 bp were appeared from day 9 to 15. On day 14, 15, 16 and 20 days of post inoculum the DNA bands were showed uniformity in the molecular weight of 650bp and it was encountered as 296, 650 and 910 bp an days 17, 18, 19, and 21 respectively in the L. wurdemanni exposed to WSSV by oral route and the WSSV appeared more virulent than in other penaeid species. There was no evidence of positive band observed in the unexposed L. wurdemanni. Mortality of 90.0 and 93.30% were noticed in the L. wurdemanni shrimps treated oral intramuscular route of WSSV inoculum and was observed on 18 and 20 days. Nevertheless, a drastic change in the survival from 100 to 40.0 and 63.30% was noticed. The day of experiment continued up to 21 days and on day 22 the experiment was terminated. Present study highlights the urgent need to adopt management measures such as screening of polychaete worms for WSSV using 2-step PCR, before use as a shrimp broodstock feed in hatchery sector.