GenBank New holotype for Enterobacter cloacae subsp. cloacae strain PR-4 isolated and identified by 16S rDNA gene sequence with Phylogenetic tree view, from explosive laden soil

The discovery of novel bacteria with their 16S rDNA and fungi with their 18S rDNA sequencing in explosive laden soil, ribosomal deoxyribonucleic acid sequencing, polymerase chain reaction and deoxyribonucleic acid sequencing has played a pivotal role in the accurate identification of bacterial/fungal isolates. Ten different Bacterial isolates and three different Actinomycetes belongs to the genera Acinetobacter, Bacillus, Enterobacter, Enterococcus, Staphylococcus, klebsiella, Aspergillus, Coriolopsis were isolated and identified with their 16S and 18S rDNA sequences and deposited in the The GenBank Maryland USA and MycoBank Utrecht Netherlands. All the isolates were named after the discoverer P Ravikumar, will be preserved in MTCC, India. Sanger dideoxy sequencing technology was employed and the number of base pairs, the base count of A, T, G and C was also studied. To fully utilise 16S/18S rDNA sequencing of bacteria and fungi in explosive laden soils and their bioremediation, the presence of cat, ben, xplA, xplB and other biodegrading gene/s, catabolic genes and their gene cassettes are being investigated. 16S ribosomal RNA gene of Enterobacter cloacae subsp. cloacae strain PR-4, with the base count 208 a 187 c 245 g 162 t partial sequence with Accession KP261383, Version KP261383.1 GI: 758375281, bases 1–802, a novel strain present in the explosive laden soil of cracker industry was deposited in the The GenBank Maryland USA is discussed here.