Metabolic syndrome and influence of pro12ala polymorphism

The Pro12Ala polymorphism of the Peroxisome Proliferator-Activated Receptor-Gamma 2 (PPAR gene) is known to be associated with metabolic syndrome. However, studies reported in south Asian population are rare. Hence, it was thought of interest to carry out this research. The study was registered in Clinical Trial Registry of India (CTRI) and Ethical Approval was obtained from Institutional Human Ethical Committee. The target populations of the study were young adults (65 male subjects) in the age group of 18–24 years. The aim of the study was to identify the frequency of pro12ala polymorphism and investigate possible association with the presence of various parameters of metabolic syndrome. The Pro12Ala genotypes were determined by PCR-restriction fragment length polymorphism (RFLP). The parameters for metabolic syndrome were assessed based on ATP III criteria. The anthropometric measurements such as height, weight, BMI, waist hip ratio were measured. Total cholesterol, HDL cholesterol and triglycerides were determined by spectrophotometery method. LDL cholesterol was calculated by the Friedewald equation. The fasting blood glucose and blood pressure were also measured. The subjects were grouped based on BMI latest Asian cutoff. The frequencies of PPAR gamma 2 genotypes were found to be PP type, 83 Per cent; PA type, 17 Per cent; and AA type, 0 Per cent. Genotype frequencies were found to obey Hardy-Weinberg equilibrium. The various parameters of metabolic syndrome such as fasting blood glucose, waist circumference, triglycerides, HDL cholesterol and blood pressure were correlated statistically with PPAR Gamma genotype.