Semenology Laboratory, Division of Physiology, Central Sheep & Wool Research Institute, Avikanagar (Via) Jaipur, Rajasthan, 304 501
Approaches to identify optimal freezing variable are essential for achieving enhanced sperm survival after cryopreservation. The use of programmable cell freezer provides controlled low temperature cooling for getting reproduceable conditions. In the present investigation the effect of programmable freezing on cryosurvival of ram spermatozoa was studied. Ram semen was processed for freezing after initial dilution with egg yolk tris glycerol extender @ 1×109 spermatozoa per ml and asperated into 0.25 ml plastic straws. Samples were gradually cooled to 5°C, frozen @ −25°C per minute upto −125°C and stored at −196°C. Thawing was done at 50°C for 10 seconds in a water bath. The mean post-thaw motility in two replicates ranged from 73.8 to 74.3 percent indicating its suitability for obtaining higher fertility rates through artificial insemination.
