Effect of scrotal insulation on tissue cells other than spermatozoa of Buck semen

Selected local bucks of non-descript breed were subjected to experimental testicular degeneration by scrotal insulation for a period of 1, 2, 4 and 8 days and the incidence with pattern of the cells other than sperm was studied at 10-day of intervals. Before scrotal insulation the average number of these cells per million sperm ranged as shaped cells 60.25 to 145.38, round cells 14.50 to 27.50, giant cells 5.50 to 13.50, epithelial cells 885.50 to 1915.00, medusa cells 1.50 to 5.00, neutrophils 1.00 to 3.00 and lymphocytes 0.00 with total of these cells 1017.87 to 2106.37. After scrotal insulation round and giant cells increased significantly in bucks treated for 2 and more days on day 20 and 30 post-insulation, except on day 30 in bucks treated for 2 days in case of giant cells. Boat shaped cells increased significantly in bucks treated for 2 and more days on day 40 and also on day 50 in 2 and 4 days treatment category. Neutrophils increased significantly in the bucks treated for 8 days only on day 20 while lymphocytes increased significantly at two intervals i.e. day 20 and 30 post-insulation. There was no change in the incidence of epithelial and medusa cells after scrotal insulation in all the treatment groups including the longest duration treatment of 8 days.