1Department of Microbiology, College of Veterinary Science and Animal Husbandry, Sardar Krushinagar - 385 506, Gujarat
2National Morbillivirus Referral Laboratory, Division of Virology, IVRI, Mukteshwar, Nanital - 263 138, Uttarakhand
*E-mail address: csannat@rediffmail.com
Peste des petits ruminants virus was isolated from tissues and blood samples of sheep and goats following second passage in Vero cells. Cytopathic effects produced in Vero cells were characterized initially by rounding and ballooning of cells, followed by aggregation of cells, formation of fusion mass and syncytia. Cell lysis was also observed in few cases. Virus isolates were identified using s-ELISA and RT-PCR.
Goats, Isolation, Peste des petitis ruminants virus, Sheep
