Polymorphism of growth hormone BMP-15 gene in Marwari goat

A study was undertaken to investigate polymorphism in BMP-15 (Fec X^Band Fec X^G in exon-2) gene in Marwari goats by RFLP technique. Samples were collected from 60 unrelated goats of Marwari breed from different locations in Bikaner district. Genomic DNA was extracted by phenol:chloroform method and amplified using exon-2 Fec X^B and Fec X^G specific primers. The amplification was obtained at between 153 bp and 141 bp. The purified amplicons were digested with five base cutter restriction enzymes: DdeI and HinfI (5’GANTCA 3’CTNAGT). All samples showed the absence of polymorphism at the Fec X^B and Fec X^G loci of BMP-15 gene. Hence, BMP-15 gene cannot be regarded as the major gene associated with the fecundity of goats. Further investigation may be directed at other loci of BMP-15 gene or other genes, using larger sample size.