Indian Journal of Small Ruminants (The)

Necropsy of a weaner, crossbred male sheep was performed having a clinical history of anorexia and weakness. The main finding revealed severe bilateral pyelonephritis. The right kidney was highly enlarged with patchy congestion, ruptured surface and haemorrhage. The renal pelvis and calyces were highly dilated with purulent content on sagittal section. The left kidney also had almost similar lesions. On bacteriological investigation Corynebacterium pseudotuberculosis, C. renale and Proteus spp were isolated. C. pseudotuberculosis was further confirmed by polymerase chain reaction (PCR), which yielded 551 bp and 382 bp amplified products of proline iminopeptidase (PIP), and nicotinamide adenine dinucleotide phosphate (NADP) oxidoreductase, respectively. Histopathologically, lesions were most severe in the medullary regions. The tubular lumen was dilated with densely eosinophilic casts. The glomeruli were less affected in comparison to the tubules. The interstitial tissues were infiltrated with lymphocytes and plasma cells. The renal pelvis was infiltrated with neutrophils.