1Microbiology Laboratory, Animal Health Division Central Institute for Research on Goats, Makhdoom- 281 122, Uttar Pradesh
Department of Microbiology, College of Veterinary Science and Animal Husbandry Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar- 385 506, Gujarat
*E-mail address: shoorvir_singh@rediffmail.com
Online published on 5 March, 2014.
Incidence of Mycobacterium avium subspecies paratuberculosis (MAP) infection was estimated in Patanwadi sheep at Livestock Research Station, Sardarkrushinagar (Gujarat), using multiple tests. More than 50.0% of Patanwadi sheep had clinical signs (reduced appetite, diarrhoea, weight loss, emaciation, rough wool coat and leathery skin) of paratuberculosis. Of 450 sheep, 133 were screened to estimate bio-load of MAP, wherein 50.3% were positive by indigenous ELISA. Faeces of 50 sheep positive in i-ELISA were screened by microscopy and 13 (26.0%) were positive for acid fast bacilli (AFB) indistinguishable from MAP. Of 13 AFB positive faecal samples in direct microscopy, 3 (23.0%) were also positive on IS900 faecal PCR. Bio-load of MAP (bactremia) was estimated in 50 i-ELISA positive sheep by IS900 blood PCR and 8 (16.0%) were found positive. This is the first study on incidence of MAP in Patanwadi sheep.
Incidence, Mycobacterium avium subspecies paratuberculosis, Patanwadi sheep
