Indian Journal of Small Ruminants (The)
SCOPUS
  • Year: 2015
  • Volume: 21
  • Issue: 2

Polymorphism of growth hormone (GH) Gene in Vembur and Kilakarsal Breeds of Sheep

1Directorate of Research, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 051, Tamil Nadu

2Translational Research for Veterinary Biologicals, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 051, Tamil Nadu

Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai-600 007, Tamil Nadu

*E-mail address: vjeichitra@gmail.com

Online published on 8 October, 2015.

Abstract

Part of exon 2, intron 3 and part of exon 3 (422 bp), exon 4 (214 bp) and part of intron 4, exon 5 and part of 3'UTR (365 bp) of growth hormone (GH) gene in Vembur and Kilakarsal sheep were screened by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) to analyse the genetic variants. MspI-RFLP of GH gene (422, 214 and 365 bp fragments) revealed absence of polymorphism in all the samples of both the breeds studied indicating no variation exists in the studied regions of GH gene.

Keywords

Growth hormone gene, Kilakarsal, MspI-RFLP, Sheep, Vembur