Department of Veterinary Physiology, College of Veterinary Sciences and Animal Husbandry U.P. Pandit Deen Dayal Upadhayaya Pashu-Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281 001, U.P.
*E-mail address: drmukulanandvet@gmail.com
Online published on 14 March, 2016.
A study was conducted to evaluate the effect of low-density lipoproteins (LDL) on motility and path velocities of spermatozoa during freezing and thawing process in Sirohi bucks. Semen (a total of 16 ejaculates) was collected from two adult bucks using artificial vagina. After initial evaluation, semen samples of the two bucks were pooled and divided in two equal parts viz., control and treatment. Control was extended with standard glycerolated tris extender with 3% egg yolk while in treatment, egg yolk was replaced with 8% of LDL extracted from egg yolk. After dilution, the semen samples were subjected to freezing and evaluated for percent live spermatozoa and hypo osmotic swelling test (HOST) after equilibration and thawing and motility pattern (rapid, slow and non-progressive) and path velocities of spermatozoa were evaluated using a CASA. The spermatozoa exhibiting the rapid progression were significantly (P 0.01) < higher in treatment after equilibration. Significantly (P 0.05) higher values of rapid and slow < progressive spermatozoa were observed in treatment compared to control in post-thaw semen. Significantly (P 0.01) higher values for curvilinear velocity (VCL), average path velocity (VAP), < straight line velocity (VSL) and maximum amplitude-lateral head displacement (ALH) were observed in the treatment after equilibration. The result indicated that LDL has a better cryoprotective action resulting in better survival rate and motility after equilibration and thawing compared to egg yolk.
Buck, Cryopreservation, Egg yolk, Low-density lipoproteins, Semen
