PCR-RFLP analysis for exons of diacylglycerol acyltransferase-1 gene in Jamunapari goats

Diacylglycerol acyltransferase-1 (DGAT-1) gene is a strong positional candidate gene for fat content in milk and meat. Genetic polymorphism for DGAT-1 gene was carried out in blood and milk samples collected from 122 Jamunapari lactating goats by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Genomic DNA was isolated from blood samples and exon 8 (222 bp fragment) was amplified by PCR. Amplified fragment was digested with EaeI restriction endonuclease. DGAT1 locus was monomorphic on PCR-RFLP analysis. Results of the present study indicated the highly conserved nature of exon 8 of DGAT1 locus due to absence of mutation in Jamunapari goats.